il 6 elisa kit  (R&D Systems)

Journal: Physiological Reports

Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

doi: 10.14814/phy2.70891

Figure Lengend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐originated lymph vessels. (b) Effects of water intake on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake on changes in the concentration of IL‐10 in the lymph. The open column, control; the black column, water intake. The error bars represent SDs.

Article Snippet: The concentrations of cytokines in the lymph were measured using enzyme‐linked immunosorbent assay (ELISA) kits: a rat IL‐1β ELISA quantitative kit (catalog no. RLB00; R&D Systems, Minneapolis, MN, USA), an IL‐6 ELISA kit (catalog no. R6000B; R&D Systems, Minneapolis, MN, USA), and a mouse/rat IL‐10 ELISA kit (catalog no. KE20003; Rosemont, IL, USA) (Arai et al., ).

Techniques: Concentration Assay, Control

Journal: Physiological Reports

Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

doi: 10.14814/phy2.70891

Figure Lengend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of clodronate (oblique line column). (b) Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐1β in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐6 in the lymph. Effects of water intake without (white column) and with clodronate (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent SDs.

Article Snippet: The concentrations of cytokines in the lymph were measured using enzyme‐linked immunosorbent assay (ELISA) kits: a rat IL‐1β ELISA quantitative kit (catalog no. RLB00; R&D Systems, Minneapolis, MN, USA), an IL‐6 ELISA kit (catalog no. R6000B; R&D Systems, Minneapolis, MN, USA), and a mouse/rat IL‐10 ELISA kit (catalog no. KE20003; Rosemont, IL, USA) (Arai et al., ).

Techniques: Derivative Assay, Concentration Assay

Journal: Physiological Reports

Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

doi: 10.14814/phy2.70891

Figure Lengend Snippet: (a) Effects of water intake on changes in lymph volume collected over set intervals of 60 min in rat jejunum‐derived lymph vessels in the absence (white column) and presence of a MyD88 inhibitor (oblique line column). (b) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐1β in the lymph. (c) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐6 in the lymph. (d) Effects of water intake without (white column) and with a MyD88 inhibitor (oblique line column) on changes in the concentration of IL‐10 in the lymph. The error bars represent the SDs.

Article Snippet: The concentrations of cytokines in the lymph were measured using enzyme‐linked immunosorbent assay (ELISA) kits: a rat IL‐1β ELISA quantitative kit (catalog no. RLB00; R&D Systems, Minneapolis, MN, USA), an IL‐6 ELISA kit (catalog no. R6000B; R&D Systems, Minneapolis, MN, USA), and a mouse/rat IL‐10 ELISA kit (catalog no. KE20003; Rosemont, IL, USA) (Arai et al., ).

Techniques: Derivative Assay, Concentration Assay

Journal: Regenerative Therapy

Article Title: Extracellular vesicles derived from adipose-derived mesenchymal stem/stromal cells prevent synovial inflammation and attenuate cartilage degeneration in rodent osteoarthritis

doi: 10.1016/j.reth.2025.101056

Figure Lengend Snippet: Anti-Inflammatory Effects of EVson IL-1β-Stimulated Human Synovial Cells. (a–c) Synovial cells collected from healthy humans were stimulated with IL-1β to induce inflammation, in addition EVs were administered simultaneously. Samples were collected and plotted at 4 h post-administration, and the evaluation was performed using real-time PCR. Significant reductions were observed in the EVs-treated group for IL-1β , IL-6 , IL-10 , TNF-α , MMP-3 , iNOS , and TGF-β(∗p < 0.05). Statistical analyses were conducted using the Mann-Whitney, and Wilcoxontests. (d) Similarly, EVs were administered to normal human synovial cells stimulated with IL-1β. At 24 h post-administration, the protein levels of FGF-18 were evaluated using an ELISA kit. In the group stimulated with IL-1β and treated with EVs, a significant increase in FGF-18 levels was observed compared to the non-stimulated group and the group treated with IL-1β alone(∗p < 0.05). Statistical analysis was performed using the Kruskal-Wallis test.

Article Snippet: The IL-6 levels in joint fluid and serum samples were measured using a commercial ELISA kit (Rat IL-6 ELISA kit, Quantikine®; R&D Systems Inc., Minneapolis, MN, USA).

Techniques: Real-time Polymerase Chain Reaction, MANN-WHITNEY, Enzyme-linked Immunosorbent Assay

Journal: Regenerative Therapy

Article Title: Extracellular vesicles derived from adipose-derived mesenchymal stem/stromal cells prevent synovial inflammation and attenuate cartilage degeneration in rodent osteoarthritis

doi: 10.1016/j.reth.2025.101056

Figure Lengend Snippet: Evaluation of Inflammatory Cytokine Levels and Histological Changes Following Evs Treatment (a) Inflammatory cytokine (IL-6) levels in serum and joint fluid were evaluated using an ELISA kit. Serum was collected from the heart after sacrifice, while joint fluid was obtained by injecting 100 μl of PBS into the joint cavity using the wash-out method. The inflammatory cytokine levels were assessed in three groups: PBS , hyaluronic acid , and EVs. In the serum, there was a tendency for IL-6 to decrease in the exosome-treated group, whereas no significant changes were observed in the joint fluid. (b, c) Histological evaluation was performed using different scoring methods. The IFP inflammation score, which indicates the severity of synovitis, showed a significant improvement in the EVs-treated group compared with the HA-treated group at 2 weeks, corresponding to the inflammatory phase (∗p < 0.05). Proliferation and fibrosis of synovial cells were observed (arrow). In contrast, the modified Mankin score, which evaluates the combined femur and tibia, was significantly lower in the EVs-treated group than in the HA-treated group during the advanced phase of OA (∗p < 0.05). Erosion of the cartilage surface were observed (arrowhead).

Article Snippet: The IL-6 levels in joint fluid and serum samples were measured using a commercial ELISA kit (Rat IL-6 ELISA kit, Quantikine®; R&D Systems Inc., Minneapolis, MN, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Modification

Journal: Journal of Neuroimmune Pharmacology

Article Title: Melatonin Mitigates Central Sensitization and Nociplastic Pain in Spinal Cord and Dorsal Root Ganglia of FM Rat Model: Modulation of SIRT1/PGC-1α/MAPK/NF-κB Signaling

doi: 10.1007/s11481-025-10274-7

Figure Lengend Snippet: Impact of Melatonin (10 mg/kg, oral, 3 days) on spinal cord pro-inflammatory modulators ( A ) p-p38 MAPK/total p38-MAPK and ( B ) NF-κB in the FM-like model. Using one-way ANOVA and Tukey’s post-hoc test (* p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001), the mean ± SD of rats ( n = 3 for MAPK and n = 6 per group for NF-κB) is represented by each bar with a vertical line. FM: fibromyalgia model, MAPK: mitogen-activated protein kinases, MEL: Melatonin, NF-κB: nuclear factor kappa B. Impact of Melatonin (10 mg/kg, oral, 3 days) on spinal cord pro-inflammatory cytokines ( A *) TNF-α, (B*) IL-1β, and ( C *) IL-6 in the FM-like model. Using one-way ANOVA and Tukey’s post-hoc test (* p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001), the mean ± SD of rodents ( n = 6) per group is indicated by each bar with a vertical line. FM: fibromyalgia model, IL-1β: interleukin-1 beta, IL-6: interleukin-6, MEL: Melatonin, TNF-α: tumor necrosis factor

Article Snippet: In addition, ELISA kits from R&D Systems were used to quantify IL-6 (Cat.#R6000B, Minneapolis, MN, USA).

Techniques:

Journal: Journal of Neuroimmune Pharmacology

Article Title: Melatonin Mitigates Central Sensitization and Nociplastic Pain in Spinal Cord and Dorsal Root Ganglia of FM Rat Model: Modulation of SIRT1/PGC-1α/MAPK/NF-κB Signaling

doi: 10.1007/s11481-025-10274-7

Figure Lengend Snippet: Experimental study diagram. AMPA receptor: α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor; DRG: dorsal root ganglia; ELISA: enzyme-linked immunosorbent assay; FM GP: fibromyalgia group; Iba-1: binding adaptor molecule 1; IL-1β: interleukin-1 beta; IL-6: interleukin-6; MAPK: mitogen-activated protein kinases; MEL GP: Melatonin group; MT1R: Melatonin receptor 1; MT2R: Melatonin receptor 2; NF-κB: nuclear factor kappa B; NMDA: N-methyl-D-aspartate receptors; NR2B: NMDA receptor subunit 2B; OFT: open field test; PCR: polymerase chain reaction; PGC-1α: peroxisome proliferator-activated receptor gamma coactivator 1-alpha; p.o.: per oral; PSD95: postsynaptic density protein 95; RES: Reserpine; RGS: rat grimace scale; RR: rotarod; RST: Randall-Selitto mechanical threshold; s.c.: subcutaneous; SIRT1: silent information regulator sirtuin 1; TNF-α: tumor necrosis factor-alpha; TST: tail suspension test; VGLUT: vesicular glutamate transporter; VFT: von Frey test

Article Snippet: In addition, ELISA kits from R&D Systems were used to quantify IL-6 (Cat.#R6000B, Minneapolis, MN, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Polymerase Chain Reaction, Randall–Selitto Test, Suspension